Call for Supplementary applications: 2019 Joint Usage and Joint Research Programs,

Institute of Advanced Medical Sciences, Tokushima University

“(A) Joint Usage 2019” only

 

The proposed expenses should be used within the fiscal year 2019 which begins adoption day 2019 and ends on 31st March 2020.

 

1)Please refer to the 2019 Joint Usage and Joint Research Programs,

2)Download: (A) Joint Usage

3)Our research budget will support the half of research expenses up to 500,000 Japanese yens per successful proposal. Successfully selected applicants must cover the rest of research expenses.

4) Application deadline is 30th August 2019.

5)  Please convert the complete application that contains the following items to a single PDF document within two pages, and send as an e-mail attachment to <kyodo@tokushima-u.ac.jp> with the subject line: “Application for Joint Usage Joint Research Programs”

6)All successfully selected applicants will receive notification.

7)Successfully selected applicants must send us a brief report of their research activities and findings. A single PDF document that contains the report has to be sent to us via e-mail by 31st March 2020.

8)Contact Administration Office

                    Institute of Advanced Medical Sciences,

                    Tokushima University 3-18-15 Kuramoto,

                    Tokushima 770-8503, Japan

                    Telephone: +81-88-633-9420

                    E-mail: kyodo@tokushima-u.ac.jp

 

  

(A)   Joint Usage

State-of-art research facility of our Institute is open for the support of the research in the following 3 aspects. Up to 500,000 Japanese yens per successful proposal per fiscal year will be supported for the following experiments.

 

A-1. Next-generation sequencing and microarray analyses (organized by Professor Toyomasa Katagiri)

Representative costs are 840,000 yens per sample for whole human genome sequencing (Illumina Hiseq1500, high-output mode, available from 3 samples), 598,000 yens per run (6 samples) for whole human exon analysis (Illumina Hiseq1500, rapid-mode), 537,000 yens per run (6 samples) for total human RNA-seq analysis (Illumina Hiseq1500, rapid-mode), 260,000 yens per run (15 samples) for RNA-seq expression analysis, for ChIP-seq analysis of transcription factors (15 samples), 440,000 yens per run (6 samples) for ChIP-Seq analysis of histone modifications (Illumina Hiseq1500, rapid- mode), and 45,000 yens per sample for Agilent human microarray analysis (Each cost is estimated as present value (2019.06). NGS analysis may be carried out for hereditary disease samples. CLC genomics workbench (Qiagen)is available in each analysis.

Each analysis will be analyzed using NextSeq550 (illumine from October 2019. Representative cost are 198,000 yens (Midi-output) or 472,000 yens (high-output) per run (3-12 samples) for whole human exon analysis, 198,000 yens (Midi) or 472,000 yens (High) per run (3-12 samples) for total human RNA-seq analysis, 255,000 yens per run (20 samples) for RNA-seq expression analysis and for ChIP-seq analysis of transcription factors (20 samples) and 198,000 yens (Midi) or 472,000 yens per run (3-12 samples) for ChIP-seq of histone modifications (The cost of Next-Seq 550 analysis  is only tentative, and may change in the future).

Please contact Professor Katagiri for details (tkatagi@genome.tokushima-u.ac.jp). Human sample analyses require the prior approval from appropriate intramural committee of the applicant’s affiliated organization.

 

A-2. Proteome analysis (organized by Professor Hidetaka Kosako)

Mass spectrometry (Orbitrap Fusion and Q Exactive Plus, Thermo Fisher Scientific) and 2D-DIGE (two-dimensional difference gel electrophoresis, GE Healthcare). Proteome Discoverer, BioPharma Finder, Mascot, Scaffold, SIEVE, Pinpoint can be used for data analysis. Representative costs are 15,000 yen per sample for identification and quantitation of proteins from electrophoresed gel fragments or immunoprecipitates, 20,000 to 30,000 yen per sample for identification and quantitation of PTMs such as phosphorylation and ubiquitination of proteins, 15,000 to 30,000 yen per sample for global identification and quantitation (by TMT labeling, PRM, and LFQ) of  proteins and PTM sites in complex samples such as cell lysates, and 15,000 yen per sample for 2D-DIGE analysis. Please contact Professor Kosako for details (kosako@tokushima- u.ac.jp). Human sample analysis requires the prior approval from appropriate intramural committee.

 

A-3. Genome editing of laboratory mice (organized by Professor Tatsuya Takemoto) Generation  of   genetically  modified  mice  by   an  improved   zygote   electroporation technology of CRISPR/Cas9-mediated genome editing. Costs are 400,000 yen for generating a gene-disrupted mouse and 600,000 yen for a mouse carrying knock-in or point mutation. Costs for mouse breeding and shipping are also required. Please contact Professor Takemoto for details (takemoto.tatsuya@tokushima-u.ac.jp)